About the facility
IVMSU is a national infrastructure for two-photon microscopy that supports intravital and non-linear light microscopy with open access to national and international users.
On this page you can learn more about our facility. Do not hesitate to reach out to us if you have any questions.
Accessibility
Web-based application via the NMI portal http://www.nmisweden.se.
Access based on
- Feasibility
- Capacity
- Recommended general guidelines from the national steering committee
Requirement for ethical permit from local ethical committee for in vivo-studies.
For BSL2 studies: Risk assessment for biological agent and toxins.
IVMSU units
- Imaging room
- Surgery lab
- Facilities for maintaining in-vivo-models
Support and service
- Microscopy- assistance and training
- Project discussion and study design
- Surgery- assistance and training
- Support for the preparation of ethical permits
- Assistance with transfer of applications/permits to the Stockholm University facility permit
- Usage of cell lab equipment (incubator and laminar flow cabinet)
- Animal handling in quarantine facility and animal housing room
- Documentation, logging and overview of in vivo-models in database system
- Possibilities for image analysis at the nearby IFSU facilities (LAS-X and Imaris)
- Access to SU-based data storage
User fees
We support feasibility tests free of charge.
For more information, please contact IVMSU@su.se.
International users, please contact IVMSU@su.se.
User fees IVMSU, academic user |
SEK |
Cage fee, price per cage and day |
|
Special cages in IVMSU*) | *) |
Usage of the multiphoton microscope for feasibility study |
|
For new users to test feasibility | free |
Usage of the Leica SP8 microscope |
|
Per hour intravital | 500 |
Maximum fee intravital per over night session kl.20-8 | 2000 |
Per hour in vitro live/ FLIM | 300 |
Maximum fee in vitro live/FLIM per over night session kl.20-8 | 1200 |
Per hour in vitro confocal | ****) |
Maximum fee in vitro confocal per over night session kl.20-8 | ****) |
Assistance, Service |
|
Assisted microscopy, per hour**) intravital | 1000 |
Assisted microscopy, per hour**) in vitro | 800 |
Surgery service, implantation of imaging window, per window ***) | 500 |
Extra animal technician service, according ECFs user fees | |
Training |
|
Microscopy intravital; 4 sessions à 2h | 4000 |
Microscopy in vitro; 2 sessions à 2h | 2000 |
Surgery, per user and imaging window, 2 sessions ***) | 2000 |
Surgery Lab |
|
Surgery lab booking per hour | 100 |
Dissection microscope per hour | 75 |
Surgery assistance, per hour | 500 |
*) according to ECF's user fees
**) intravital 500 Kr/h microscopy+ 500Kr/h assistance; in vitro 300Kr/h microscopy+ 500Kr/h assistance
***) abdominal/subcutaneous window, including rental material, not including disposables and animals costs
****) according to IFSU’s fees for confocal imaging
Projects
Ongoing projects*
Multicolor cell labeling for solving the clonal structure of murine embryo neural crest
Department of Medical Bioschemistry and Biophysics, Karolinska Institutet
In this project, we will experimentally target clonal structures of the cranial neural crest cells and will study their reparative plasticity as well as underlying signaling mechanisms that can be considered as new therapeutic targets. At the single cell and clonal levels, we will systematically explore the spectrum of signals integrating the growing oral and craniofacial complex.
The effect of temperature on the bumblebee brain
Department of Zoology, Stockholm University
The aim of the project is to understand if and how the information carried by neurons in the bumblebee brain is affected by temperature. The findings will have consequences for understanding how increasing environmental temperatures, such as those that are occurring as a result of climate change, will affect the function of insect nervous systems and behavior.
Evaluation of autofluorescence life-time imaging to observe differences in biological samples
Evaluation of autofluorescence life-time imaging to observe differences in biological samples
Department of Ecology, Environment and Plant Sciences, Stockholm University
Imaging Candida albicans infection in the mouse kidney
Department of Molecular Biosciences, Stockholm University
*All in vivo projects are performed in compliance with ethics permits approved by the local ethics committee.
Finished projects*
- FRET-FISH: combining DNA FISH with FLIM-FRET for quantification of chromosome X inactivation in mouse. Department of Physiology and Pharmacology, Karolinska Institutet
- Live monitoring of cellular dynamics during skeletal development and maintenance. Department of Physiology and Pharmacology, Karolinska Institutet.
- Second harmonic generation imaging of collagen gels. Department of Micro and Nanosystems, KTH Royal Institute of Technology, Sample: hydrogel based on type I collagen
- Determining the effect of Piezo1 activation on membrane tension in cancer cells. Department of Medical Cell Biology, Uppsala University. Samples: MDA-MB-231 breast cancer cells
- Real-time visualisation of human stem cells during neuronal differentiation. Department of Organismal Biology, Uppsala University. Samples: Human embryonic stem cells.
*All in vivo projects are performed in compliance with ethics permits approved by the local ethics committee.
Publications
Blood-brain barrier-restricted translocation of Toxoplasma gondii from cortical capillaries. Gabriela C. Olivera, Emily C. Ross, Christiane Peuckert, Antonio Barragan. Elife. 2021;10:e69182.doi: 10.7554/eLife.69182.
Three Dimensional Microvascularized Tissue Models by Laser-Based Cavitation Molding of Collagen. Alessandro Enrico, Dimitrios Voulgaris, Rebecca Östmans, Naveen Sundaravadivel, Lucille Moutaux, Aurélie Cordier, Frank Niklaus, Anna Herland, and Göran Stemme. Adv Mater. 2022;e2109823.doi: 10.1002/adma.202109823
FRET-FISH probes chromatin compaction at individual genomic loci in single cells. Mota A, Berezicki S, Wernersson E, Harbers L, Li-Wang X, Gradin K, Peuckert C, Crosetto N, Bienko M. Nat Commun. 2022 Nov 5;13(1):6680. doi: 10.1038/s41467-022-34183-y.
Last updated: December 12, 2022
Source: MBW