Experimental Chemical Methods
Experimental Chemical Methods’ will introduce you to a number of important methods used in chemistry, biochemistry and molecular biology
The theoretical part of the course, which consist of a series of lectures, will be supplemented with computer labs and instrument demonstrations
‘Experimental Chemical Methods’ will introduce you to a number of important methods used in chemistry, biochemistry and molecular biology. The theoretical part of the course, which consist of a series of lectures, will be supplemented with computer labs and instrument demonstrations.
- Explain the theory behind diffraction, microscopy, spectroscopy, mass-cytometry, and DNA sequencing.
- Explain what scientific questions the methods can answer, and describe the strengths and weaknesses of a technique for particular applications.
You will be expected to:
- Attend and actively participate in the lectures, labs and demonstrations.
- Complete the assignments and lab reports on time.
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Course structure
- Next generation sequencing: This part consists of lectures on the principles of “next-generation sequencing” (NGS), and how NGS can be used to study a range of chemical and biological questions, and an overview of the computational tools (bioinformatics) used to explore and interpret NGS data. The theoretical part is complemented with a computer lab to try some of the bioinformatics tools.
- Mass spectrometry. The lectures on mass spectrometry is accompanied by a demo lab.
- Diffraction and Cryo-EM. This part consists of lectures on diffraction-based, as well as, cryo-electron microscopy based structural biochemistry. The lectures are complemented with computer labs and lab demonstrations.
- Fluorescence microscopy. The lecture on fluorescence microscopy and live-cell imaging is complemented with a computer lab on quantitative image analysis.
- NMR and light spectroscopy. This part consists of only lectures.
Teaching format
Lectures, seminars and lab work
Assessment
Written exam, home exams, and lab reports.
Examiner
Mats Nilsson
Science for Life Laboratory
Department of Biochemistry and Biophysics
Stockholm University
Sweden
Email: mats.nilsson@dbb.su.se or mats.nilsson@scilifelab.se -
Schedule
The schedule will be available no later than one month before the start of the course. We do not recommend print-outs as changes can occur. At the start of the course, your department will advise where you can find your schedule during the course. -
Contact
Mats Nilsson
Email: mats.nilsson@dbb.su.se or mats.nilsson@scilifelab.seChemistry Section & Student Affairs Office:
Office: Chemical Practice Laboratory M345
E-mail: chemistry@su.se
